17603   Dnajc14     Status: ES cell colonies screened / QC positives

GeneDnajc14
Chromosome10
Deletion Start128,817,428
Deletion End128,806,214
Deletion Size11,215
Genome Build38
MGI IDMGI:1921580
NCBI Gene ID74330
BAC ID265A21
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 10

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF128,806,985128,807,009CTTCTGGTATTGGTGGGAGAGTATG
TUP128,807,010128,807,037TGGAAACCTGTGGCCATCTCATTTACGC
TUR128,807,050128,807,069GGTCCAGGTCACTGCCTTTC
TDF128,817,286128,817,306AACTTCTTTGCCGCACCTCAC
TDP128,817,308128,817,329CTGGCCCTGGGACCACTTCGAC
TDR128,817,359128,817,379GCCGTTTAGGTTTGGCTTCTC

PCR Primers

PrimerSequence
SUCCTCCCTCTTTCAGGCAGTC
SDAGTTCCCACACCTCAGAAGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR85 bp++-
TDFTDR94 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17603A-A2VGB6C57BL/6NTac
17603A-A10VGB6C57BL/6NTac
17603A-C4VGB6C57BL/6NTac
17603A-C7VGB6C57BL/6NTac
17603A-C11VGB6C57BL/6NTac
17603A-G2VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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