| Gene | Dpp9 |
| Chromosome | 17 |
| Deletion Start | 56,211,799 |
| Deletion End | 56,187,296 |
| Deletion Size | 24,504 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,210,962 | 56,210,984 | CAGTTAGGCTTGTTTGCCGAGAG |
| TUP | 56,210,935 | 56,210,961 | CGTGATCCTGGCTGCTGTCTAGAGGTA |
| TUR | 56,210,902 | 56,210,922 | GAGATCCAGCCGAGTCCATAG |
| TDF | 56,188,185 | 56,188,203 | AGCTCCGAAGGGTCCTGTG |
| TDP | 56,188,161 | 56,188,183 | TCAGTCACTGACCAGCCTGTGCC |
| TDR | 56,188,109 | 56,188,130 | GGGTAGTCACTGAGGTGTTGTC |
PCR Primers
| Primer | Sequence |
| SU | GTCTGGAAGTCACAGGAGGG |
| SD | TCGGTACCGCTTACTTCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17604A-B5 | VGB6 | C57BL/6NTac | |
| 17604A-C10 | VGB6 | C57BL/6NTac | |
| 17604A-D3 | VGB6 | C57BL/6NTac | |
| 17604A-E1 | VGB6 | C57BL/6NTac | |
| 17604A-G1 | VGB6 | C57BL/6NTac | |
| 17604A-E2 | VGB6 | C57BL/6NTac | |
