| Gene | E130303B06Rik |
| Chromosome | 8 |
| Deletion Start | 105,707,905 |
| Deletion End | 105,703,768 |
| Deletion Size | 4,138 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,707,752 | 105,707,770 | GGCTTTCCACCGCGTCTTG |
| TUP | 105,707,730 | 105,707,750 | TCCTCAGCCCAAGGACGCCTG |
| TUR | 105,707,697 | 105,707,718 | AGTCAGCAGGTCCAGCTTCAAC |
| TDF | 105,704,413 | 105,704,433 | CAGGTCCTTGCACAGGTTCAG |
| TDP | 105,704,391 | 105,704,411 | AACAGCAGCGCCAAGCCCAGG |
| TDR | 105,704,366 | 105,704,386 | CCTTTCTGCGTGGCGTTGTAG |
PCR Primers
| Primer | Sequence |
| SU | TCGCCTTAGTCATTTGACCC |
| SD | GCAGCCCTTGTCACTTTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17606A-A5 | VGB6 | C57BL/6NTac | |
| 17606A-C7 | VGB6 | C57BL/6NTac | |
| 17606A-C11 | VGB6 | C57BL/6NTac | |
| 17606A-G1 | VGB6 | C57BL/6NTac | |
| 17606A-D9 | VGB6 | C57BL/6NTac | |
| 17606A-D5 | VGB6 | C57BL/6NTac | |
