| Gene | Elmod3 |
| Chromosome | 6 |
| Deletion Start | 72,594,790 |
| Deletion End | 72,566,608 |
| Deletion Size | 28,183 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,594,094 | 72,594,114 | AAAGGAGTCTGGAGGCAGGAG |
| TUP | 72,594,070 | 72,594,092 | TAAAGCAGCCGTGGAGGAGCACT |
| TUR | 72,594,021 | 72,594,043 | AGAAGAAAGAGGCTGGATGTGAG |
| TDF | 72,566,875 | 72,566,895 | AAGCCTTCATTCCCTGCTCTC |
| TDP | 72,566,846 | 72,566,867 | CCAGACTTGGAAGCGTTGGCCA |
| TDR | 72,566,795 | 72,566,813 | CCTGGGCGAGGTAGTCATC |
PCR Primers
| Primer | Sequence |
| SU | CCAGTCCCTCTACTCCAC |
| SD | GAATCTTGGCTGTTGCTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17608A-H12 | VGB6 | C57BL/6NTac | |
| 17608A-D7 | VGB6 | C57BL/6NTac | |
| 17608A-H3 | VGB6 | C57BL/6NTac | |
| 17608A-B8 | VGB6 | C57BL/6NTac | |
