| Gene | Ethe1 |
| Chromosome | 7 |
| Deletion Start | 24,608,787 |
| Deletion End | 24,593,934 |
| Deletion Size | 14,854 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,594,100 | 24,594,119 | TCCTTCATGCCTGGCTGTTG |
| TUP | 24,594,122 | 24,594,150 | AAACAAGGAGTCCCGACCCTTTAACAGGG |
| TUR | 24,594,152 | 24,594,172 | GCTCAGGGTCCCACTAACTAC |
| TDF | 24,608,021 | 24,608,040 | CCTCTGCCTGTCGTTCTCAC |
| TDP | 24,608,042 | 24,608,064 | AGGCATGGAGGCAACAGAACCCA |
| TDR | 24,608,094 | 24,608,113 | ATCCACTCAGCACGCCCAAG |
PCR Primers
| Primer | Sequence |
| SU | CCGTGGCTCTAAGTGTCTAG |
| SD | TTTGAGAAACCAGGGCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17609A-A1 | VGB6 | C57BL/6NTac | |
| 17609A-B12 | VGB6 | C57BL/6NTac | |
| 17609A-C1 | VGB6 | C57BL/6NTac | |
| 17609A-C3 | VGB6 | C57BL/6NTac | |
| 17609A-H10 | VGB6 | C57BL/6NTac | |
| 17609A-G10 | VGB6 | C57BL/6NTac | |
