| Gene | Fam122c |
| Chromosome | X |
| Deletion Start | 53,301,389 |
| Deletion End | 53,277,622 |
| Deletion Size | 23,768 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,277,732 | 53,277,751 | ACCAGCTTCCAGCTCTGTAG |
| TUP | 53,277,756 | 53,277,785 | CAGCTACGTTAGAAGATCCAACAGCATCCC |
| TUR | 53,277,796 | 53,277,815 | TACCCTGCTCACCCAAATCC |
| TDF | 53,300,390 | 53,300,411 | TCCGACTGGCCTTTCATTTGTC |
| TDP | 53,300,417 | 53,300,438 | CTTGACTTGACCTCCTGAATAG |
| TDR | 53,300,452 | 53,300,474 | TCATTGCCACTTAGCCTGATAGC |
PCR Primers
| Primer | Sequence |
| SU | CATAAGTGCCCTGTTGCCTC |
| SD | GAGTATTCATGTATGAACTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17613A-A4 | VGB6 | C57BL/6NTac | |
| 17613A-E7 | VGB6 | C57BL/6NTac | |
| 17613A-F7 | VGB6 | C57BL/6NTac | |
| 17613A-H2 | VGB6 | C57BL/6NTac | |
