| Gene | Fam178b |
| Chromosome | 1 |
| Deletion Start | 36,660,767 |
| Deletion End | 36,632,423 |
| Deletion Size | 28,345 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,660,701 | 36,660,720 | GGCCATCTTGGCTTCTCCTC |
| TUP | 36,660,673 | 36,660,698 | TGGCCTCTCCTTTGAGTGACCTCTTA |
| TUR | 36,660,634 | 36,660,654 | GCAGAAAGGGCCATCAGAAAG |
| TDF | 36,633,215 | 36,633,235 | CCAGGTGAACAGCCCATTCTG |
| TDP | 36,633,186 | 36,633,214 | AGCAAGTGATTCATTGCCATCAGACTCCA |
| TDR | 36,633,090 | 36,633,110 | TCTAGGCTCAGCTAGGATGAC |
PCR Primers
| Primer | Sequence |
| SU | TCTTTGCAGAGCCTCAAGTG |
| SD | GGCAGAACTCACCTTCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 146 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17614A-A9 | VGB6 | C57BL/6NTac | |
| 17614A-C1 | VGB6 | C57BL/6NTac | |
| 17614A-C9 | VGB6 | C57BL/6NTac | |
| 17614A-G10 | VGB6 | C57BL/6NTac | |
| 17614A-G5 | VGB6 | C57BL/6NTac | |
| 17614A-B1 | VGB6 | C57BL/6NTac | |
