| Gene | Fam53a |
| Chromosome | 5 |
| Deletion Start | 33,614,677 |
| Deletion End | 33,600,558 |
| Deletion Size | 14,120 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,614,384 | 33,614,406 | TCGGCCAGAGTAAGTAAGTCATC |
| TUP | 33,614,360 | 33,614,383 | CTGTGATCCTGCTACCCGTGTCCA |
| TUR | 33,614,340 | 33,614,359 | CAAATTGTGCGACAGGGTTC |
| TDF | 33,601,022 | 33,601,043 | TGATGAGGCTAGGGATGAGATG |
| TDP | 33,600,993 | 33,601,019 | CAGAGTCCAGGAAACAGTTCTAGGGCA |
| TDR | 33,600,970 | 33,600,988 | CCCAGTCACCAGACACCAG |
PCR Primers
| Primer | Sequence |
| SU | AAGGCATTTGTCAAAGAGGG |
| SD | TCAATGGAAAGGAAACCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17615A-F8 | VGB6 | C57BL/6NTac | |
| 17615A-C5 | VGB6 | C57BL/6NTac | |
| 17615A-D2 | VGB6 | C57BL/6NTac | |
| 17615A-E8 | VGB6 | C57BL/6NTac | |
| 17615A-E4 | VGB6 | C57BL/6NTac | |
| 17615A-A4 | VGB6 | C57BL/6NTac | |
