| Gene | Fbxo28 |
| Chromosome | 1 |
| Deletion Start | 182,341,586 |
| Deletion End | 182,316,824 |
| Deletion Size | 24,763 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 182,340,761 | 182,340,780 | CCAGAGCCTTAGTTGCTCAC |
| TUP | 182,340,730 | 182,340,755 | TAGAGCCCTGGGAGATGATGACCTGA |
| TUR | 182,340,706 | 182,340,725 | GGAGTGTCAGGGAGGCAAAG |
| TDF | 182,317,059 | 182,317,080 | GACCCAGATCATAGGCGAACAG |
| TDP | 182,317,039 | 182,317,058 | AACGCACGGCTGGCAGAGCT |
| TDR | 182,316,999 | 182,317,022 | CCCTACTGCACTTTCCATGACTTC |
PCR Primers
| Primer | Sequence |
| SU | AACTGCTCCATTGTCGTTCC |
| SD | TCTCAGACATTCCAGACAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17616A-A8 | VGB6 | C57BL/6NTac | |
| 17616A-B10 | VGB6 | C57BL/6NTac | |
| 17616A-C7 | VGB6 | C57BL/6NTac | |
| 17616A-E5 | VGB6 | C57BL/6NTac | |
| 17616A-F7 | VGB6 | C57BL/6NTac | |
| 17616A-G2 | VGB6 | C57BL/6NTac | |
