| Gene | Gfod2 |
| Chromosome | 8 |
| Deletion Start | 105,728,243 |
| Deletion End | 105,716,689 |
| Deletion Size | 11,555 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,728,028 | 105,728,048 | GACCTGGTCTGCATCAACATC |
| TUP | 105,728,003 | 105,728,026 | CACCTCCGCTCACCCGGCAGATAT |
| TUR | 105,727,939 | 105,727,957 | TGCCCAGAGGAGGAAACAC |
| TDF | 105,717,339 | 105,717,359 | ACCTGTTGACCCACTTGACTG |
| TDP | 105,717,307 | 105,717,329 | CCGAGAAGGTGCACGGGCTCCTC |
| TDR | 105,717,272 | 105,717,291 | CCACGGATGGTGGCATTCTG |
PCR Primers
| Primer | Sequence |
| SU | CTGTGTCCAGTAGGTGACGG |
| SD | TTCTCCAGTCCTGAGATGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17621A-A1 | VGB6 | C57BL/6NTac | |
| 17621A-H12 | VGB6 | C57BL/6NTac | |
| 17621A-B6 | VGB6 | C57BL/6NTac | |
| 17621A-C2 | VGB6 | C57BL/6NTac | |
| 17621A-H3 | VGB6 | C57BL/6NTac | |
| 17621A-F4 | VGB6 | C57BL/6NTac | |
