| Gene | Ggt1 |
| Chromosome | 10 |
| Deletion Start | 75,586,124 |
| Deletion End | 75,574,239 |
| Deletion Size | 11,886 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,574,402 | 75,574,420 | TGGGTCTGCTAGGGCAAGG |
| TUP | 75,574,421 | 75,574,441 | CACCCTGGAAGGCCAGTGAGC |
| TUR | 75,574,492 | 75,574,509 | AAAGGACGGGCAGCTCAC |
| TDF | 75,585,905 | 75,585,923 | CGGCACCACCATACAGAAG |
| TDP | 75,585,925 | 75,585,946 | CACGCCCACCTTCATCGCTGTA |
| TDR | 75,585,947 | 75,585,964 | TCGAACGACAGCCTGAAC |
PCR Primers
| Primer | Sequence |
| SU | GAGTCTGACCGGTCTCCTTG |
| SD | ATGAGAGCCCACATCTGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 60 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17622A-A4 | VGB6 | C57BL/6NTac | |
| 17622A-A6 | VGB6 | C57BL/6NTac | |
| 17622A-A7 | VGB6 | C57BL/6NTac | |
| 17622A-D8 | VGB6 | C57BL/6NTac | |
| 17622A-F8 | VGB6 | C57BL/6NTac | |
| 17622A-H11 | VGB6 | C57BL/6NTac | |
