| Gene | Golga1 |
| Chromosome | 2 |
| Deletion Start | 39,063,373 |
| Deletion End | 39,034,065 |
| Deletion Size | 29,309 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 39,063,321 | 39,063,341 | GACTGCTGTTGCTCAGAGACC |
| TUP | 39,063,296 | 39,063,317 | CGGCACAACAAGGATCCCACGC |
| TUR | 39,063,273 | 39,063,292 | GCCACAGACTCCTTGCTCAC |
| TDF | 39,034,149 | 39,034,168 | CTTGCCCTGGAACAGCATTG |
| TDP | 39,034,126 | 39,034,148 | CAGGGAGCGCATCCATGCACTGG |
| TDR | 39,034,065 | 39,034,084 | CCCTGCTCTGAGGCTATCTG |
PCR Primers
| Primer | Sequence |
| SU | TTCTGCCTCCTTCAGACCAG |
| SD | TAGAGATCAGGGAGTTGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17626A-G12 | VGB6 | C57BL/6NTac | |
| 17626A-H7 | VGB6 | C57BL/6NTac | |
