| Gene | Grin1 |
| Chromosome | 2 |
| Deletion Start | 25,318,883 |
| Deletion End | 25,292,022 |
| Deletion Size | 26,862 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,318,265 | 25,318,286 | CATGGGTCTGCATAGGATGGTC |
| TUP | 25,318,242 | 25,318,264 | CAGGTGCCAGAGCAAACAGGCTC |
| TUR | 25,318,192 | 25,318,211 | TCTGTGCCTTGGTGTCTCTG |
| TDF | 25,292,801 | 25,292,821 | GGCCTTGCTCTCAGTTCAATC |
| TDP | 25,292,779 | 25,292,800 | TTTACCTGAGCCTCCCTGGGCA |
| TDR | 25,292,747 | 25,292,765 | GCTCCAGATGGCCCAGAAG |
PCR Primers
| Primer | Sequence |
| SU | CTCCAAGCATTTACGCCAAC |
| SD | CTGCCATTTCTGCTCCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17627A-C7 | VGB6 | C57BL/6NTac | |
| 17627A-D7 | VGB6 | C57BL/6NTac | |
| 17627A-G9 | VGB6 | C57BL/6NTac | |
| 17627A-H10 | VGB6 | C57BL/6NTac | |
