| Gene | Hmox2 |
| Chromosome | 16 |
| Deletion Start | 4,766,106 |
| Deletion End | 4,762,598 |
| Deletion Size | 3,509 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,762,730 | 4,762,752 | GTCAGTGTGCCTGATTCAGTATG |
| TUP | 4,762,757 | 4,762,784 | AGGAAATGCTCATCAGCACTGAGCTGTA |
| TUR | 4,762,833 | 4,762,852 | AGCCAGTGACAGGACTCTAC |
| TDF | 4,765,736 | 4,765,757 | TGGCCTCTGATGACTTCTGTTC |
| TDP | 4,765,763 | 4,765,789 | ATATTCAGTGAACTGGACCAGGCTGGC |
| TDR | 4,765,803 | 4,765,822 | CCCATCCTCCAGGGTTTCTC |
PCR Primers
| Primer | Sequence |
| SU | TTGCATTGCTTACGAAGCTG |
| SD | CATGACCCTTTCCCACAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 123 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17628A-H3 | VGB6 | C57BL/6NTac | |
| 17628A-E5 | VGB6 | C57BL/6NTac | |
| 17628A-F11 | VGB6 | C57BL/6NTac | |
| 17628A-E10 | VGB6 | C57BL/6NTac | |
| 17628A-F8 | VGB6 | C57BL/6NTac | |
| 17628A-C4 | VGB6 | C57BL/6NTac | |
