| Gene | Hsp90ab1 |
| Chromosome | 17 |
| Deletion Start | 45,571,908 |
| Deletion End | 45,567,949 |
| Deletion Size | 3,960 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,571,248 | 45,571,272 | TCTGGACATTGAACTGATTGTTACC |
| TUP | 45,571,222 | 45,571,247 | TTGCGCAGCAGATGGTTATACTTGGC |
| TUR | 45,571,199 | 45,571,221 | GAGGATCAGCAACATGGTTCTTG |
| TDF | 45,568,634 | 45,568,653 | TCCTCTGCCTGCCATCTTAG |
| TDP | 45,568,591 | 45,568,620 | ACATAGCAGTTCCATTATGGCCAAGGTACA |
| TDR | 45,568,565 | 45,568,589 | GTGGACAATCAAGAACAATGACAAG |
PCR Primers
| Primer | Sequence |
| SU | GGTGTTCTGTTCAGAGTCAG |
| SD | CCAGTGAGAATGGTGCTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17630A-A1 | VGB6 | C57BL/6NTac | |
| 17630A-H12 | VGB6 | C57BL/6NTac | |
| 17630A-D3 | VGB6 | C57BL/6NTac | |
| 17630A-G6 | VGB6 | C57BL/6NTac | |
| 17630A-F8 | VGB6 | C57BL/6NTac | |
