Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

17631 Ift20 Status: ES cell colonies screened / QC positives

Gene	Ift20
Chromosome	11
Deletion Start	78,541,538
Deletion End	78,538,839
Deletion Size	2,700
Genome Build	38

MGI ID	MGI:1915585
NCBI Gene ID	55978
BAC ID	53O16
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	78,538,931	78,538,950	GCTCAAGGAGGAGTGCAAGG
TUP	78,538,957	78,538,984	TGGACAGTGAGTACCATCTGGAGACACA
TUR	78,539,005	78,539,025	GCCAAGGTCAGGTAAGGAAAC
TDF	78,540,737	78,540,755	ACACTTGTGGGCCTGACTG
TDP	78,540,757	78,540,781	TCGTGCTGCCATTTCGTACCAGGTA
TDR	78,540,788	78,540,812	CATGACTATTAACCTCCTCTTCTCC

PCR Primers

Primer	Sequence
SU	AGGCCAGATACACCCAAGTG
SD	CTGGTCCAAATAAAGGCAGC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	95 bp	+	+	-
TDF	TDR	76 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
17631A-C5	VGB6	C57BL/6NTac
17631A-D12	VGB6	C57BL/6NTac
17631A-G6	VGB6	C57BL/6NTac
17631A-H2	VGB6	C57BL/6NTac