| Gene | Ing3 |
| Chromosome | 6 |
| Deletion Start | 21,973,797 |
| Deletion End | 21,949,769 |
| Deletion Size | 24,029 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,950,267 | 21,950,287 | TGCATGGAGATGACGACACTG |
| TUP | 21,950,288 | 21,950,311 | CACCGCAGTGGAAACTCGCGTGAT |
| TUR | 21,950,315 | 21,950,334 | GCTCGGGTGCAAACCTTCTG |
| TDF | 21,973,406 | 21,973,427 | GAGGCACATCTGTCAGACTCAG |
| TDP | 21,973,429 | 21,973,453 | AACGTTTAGTCACTGCCAGGGTCCA |
| TDR | 21,973,460 | 21,973,484 | ACCAAACTTAGGTCTGTGCGATCAG |
PCR Primers
| Primer | Sequence |
| SU | CATACAACTCCCGTCATCCC |
| SD | TCTTCATCGTTGGAATTCTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17632A-B5 | VGB6 | C57BL/6NTac | |
| 17632A-C7 | VGB6 | C57BL/6NTac | |
| 17632A-B9 | VGB6 | C57BL/6NTac | |
