| Gene | Ipo4 |
| Chromosome | 14 |
| Deletion Start | 55,635,595 |
| Deletion End | 55,625,886 |
| Deletion Size | 9,710 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,635,050 | 55,635,068 | GCCTTTGCCACCTGTCTTC |
| TUP | 55,635,024 | 55,635,049 | CAGCCTCAAGTCCCTGGTCCTGACAG |
| TUR | 55,634,992 | 55,635,012 | GCTCCACACTTACACTGTCTC |
| TDF | 55,626,305 | 55,626,327 | TGTTGGGTGTCAGTTCAGACAAG |
| TDP | 55,626,275 | 55,626,302 | CACAGGCTTCTGTCCTTGGTTTCAACGG |
| TDR | 55,626,207 | 55,626,228 | GAAGCCACATCCACAACCTATG |
PCR Primers
| Primer | Sequence |
| SU | ACACAAACTCTGGACTCTGG |
| SD | AGATGTGCAAGGGAATGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 121 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17634A-C8 | VGB6 | C57BL/6NTac | |
| 17634A-E1 | VGB6 | C57BL/6NTac | |
| 17634A-H5 | VGB6 | C57BL/6NTac | |
| 17634A-F9 | VGB6 | C57BL/6NTac | |
| 17634A-F6 | VGB6 | C57BL/6NTac | |
