| Gene | Jmjd4 |
| Chromosome | 11 |
| Deletion Start | 59,455,691 |
| Deletion End | 59,450,248 |
| Deletion Size | 5,444 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,450,884 | 59,450,903 | AGCAGCTAGTGCAGCCAGTC |
| TUP | 59,450,905 | 59,450,925 | AGGTGGAGCACGCCCACTGCA |
| TUR | 59,450,970 | 59,450,990 | CCCACATCTTCGCATCAGTTC |
| TDF | 59,455,448 | 59,455,470 | CAGCAGGCTGCATTTGATATTGG |
| TDP | 59,455,472 | 59,455,493 | CGCCTTGCAGATGTGCTGGCCT |
| TDR | 59,455,494 | 59,455,517 | GAAAGTCAGGATTCACAACCACAG |
PCR Primers
| Primer | Sequence |
| SU | CTCACCTGAGGCAAGAGGAG |
| SD | TGAGAGTCTTGGAGGGCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17636A-A12 | VGB6 | C57BL/6NTac | |
| 17636A-G1 | VGB6 | C57BL/6NTac | |
| 17636A-B11 | VGB6 | C57BL/6NTac | |
| 17636A-C12 | VGB6 | C57BL/6NTac | |
| 17636A-E4 | VGB6 | C57BL/6NTac | |
