| Gene | Klc2 |
| Chromosome | 19 |
| Deletion Start | 5,117,418 |
| Deletion End | 5,108,590 |
| Deletion Size | 8,829 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,117,154 | 5,117,175 | TGTGGTGGAAGATTACCCGAAG |
| TUP | 5,117,124 | 5,117,148 | TGGTGATGTAGGAACACCCTCAGCA |
| TUR | 5,117,093 | 5,117,112 | AACGTGACGCTCTGAAAGAC |
| TDF | 5,108,995 | 5,109,016 | CCCTGGTGAGTCTTTAGCCAAC |
| TDP | 5,108,971 | 5,108,994 | CACTGCTCTGGTTTCAGGCGGAGA |
| TDR | 5,108,944 | 5,108,964 | ACCTCTCCCTCCACTTGTTTC |
PCR Primers
| Primer | Sequence |
| SU | CTTGCAATGCCCTAGTGCTC |
| SD | GAGGGCTGAGAGGATGACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17638A-H12 | VGB6 | C57BL/6NTac | |
| 17638A-C10 | VGB6 | C57BL/6NTac | |
| 17638A-C11 | VGB6 | C57BL/6NTac | |
| 17638A-G4 | VGB6 | C57BL/6NTac | |
| 17638A-D1 | VGB6 | C57BL/6NTac | |
| 17638A-B6 | VGB6 | C57BL/6NTac | |
