| Gene | Map2k3 |
| Chromosome | 11 |
| Deletion Start | 60,951,931 |
| Deletion End | 60,932,226 |
| Deletion Size | 19,706 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,932,655 | 60,932,675 | CAATGCCGGAAACCTGTCTAC |
| TUP | 60,932,677 | 60,932,702 | CAGAGCTCCTCCACTCTTCCAACTCC |
| TUR | 60,932,706 | 60,932,729 | GGCAAAGGAGGATTCACAAGGATG |
| TDF | 60,951,132 | 60,951,153 | CCTGTCTTCACTGCCCTCTTTC |
| TDP | 60,951,156 | 60,951,180 | AGCCTAAGGAAGAACCCTGCAGAGC |
| TDR | 60,951,192 | 60,951,212 | CACACTCACCATCAGCTCCAG |
PCR Primers
| Primer | Sequence |
| SU | GAGTGGGTAGGGCAGTCATC |
| SD | GGCCACAGTGTACGAAGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17640A-A1 | VGB6 | C57BL/6NTac | |
| 17640A-D12 | VGB6 | C57BL/6NTac | |
| 17640A-C3 | VGB6 | C57BL/6NTac | |
| 17640A-B2 | VGB6 | C57BL/6NTac | |
| 17640A-C4 | VGB6 | C57BL/6NTac | |
| 17640A-F7 | VGB6 | C57BL/6NTac | |
