| Gene | Med19 |
| Chromosome | 2 |
| Deletion Start | 84,686,994 |
| Deletion End | 84,678,448 |
| Deletion Size | 8,547 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,678,649 | 84,678,669 | CGGGAATTGCCAGGTGAGTAG |
| TUP | 84,678,671 | 84,678,697 | AGGCCAAGAGTAGCCACTCAGATTGTA |
| TUR | 84,678,737 | 84,678,756 | CCGCGTAGCCCTGTTATCTC |
| TDF | 84,686,862 | 84,686,883 | CCAGAACCGACATAGTCCAGAC |
| TDP | 84,686,884 | 84,686,906 | CACCCAGGTATGGGCAGCTCTCA |
| TDR | 84,686,963 | 84,686,987 | CAGCAGGTTCAGTATCACAGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | ATATCAGATGCAACGCCTCC |
| SD | TCAGACCATGTTCTCTATTCGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 126 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17644A-B4 | VGB6 | C57BL/6NTac | |
| 17644A-B12 | VGB6 | C57BL/6NTac | |
| 17644A-D11 | VGB6 | C57BL/6NTac | |
| 17644A-F3 | VGB6 | C57BL/6NTac | |
| 17644A-F7 | VGB6 | C57BL/6NTac | |
| 17644A-H11 | VGB6 | C57BL/6NTac | |
