| Gene | Mta2 |
| Chromosome | 19 |
| Deletion Start | 8,951,666 |
| Deletion End | 8,942,358 |
| Deletion Size | 9,309 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,942,527 | 8,942,545 | TATCGGGCGCAGAGTCTAC |
| TUP | 8,942,548 | 8,942,572 | CAGAGGCTTACCTGCAGCCGTAACG |
| TUR | 8,942,578 | 8,942,599 | CGATCCAGTCACAGGTCTCAAG |
| TDF | 8,951,210 | 8,951,229 | AGCAGCCAAGCGTCAGAAAC |
| TDP | 8,951,231 | 8,951,256 | AAACCCTGCTGATGCTCCCAATCCAG |
| TDR | 8,951,277 | 8,951,295 | TCCCTCCCTACCTGGTGTC |
PCR Primers
| Primer | Sequence |
| SU | CGAGCTCGTTCCGGAAGAAG |
| SD | CGGACACCGGACAGTAAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17645A-E9 | VGB6 | C57BL/6NTac | |
| 17645A-B5 | VGB6 | C57BL/6NTac | |
| 17645A-C6 | VGB6 | C57BL/6NTac | |
| 17645A-F10 | VGB6 | C57BL/6NTac | |
| 17645A-G6 | VGB6 | C57BL/6NTac | |
