| Gene | Mtfr1 |
| Chromosome | 3 |
| Deletion Start | 19,219,140 |
| Deletion End | 19,200,105 |
| Deletion Size | 19,036 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,200,130 | 19,200,150 | GGATGTGGTTTCAGCGAGTTG |
| TUP | 19,200,154 | 19,200,177 | TCAGCATGCAGTCGGTGAGTCTCA |
| TUR | 19,200,197 | 19,200,219 | GCCTCCGAACATCTACATTTGTG |
| TDF | 19,218,506 | 19,218,525 | AGCAGGGTTGGCTTAAATCC |
| TDP | 19,218,532 | 19,218,557 | TGCTACCTCTACCTCTGATGCTGGGA |
| TDR | 19,218,563 | 19,218,584 | CTGGTCACAATGGCACAACTAC |
PCR Primers
| Primer | Sequence |
| SU | GGTTAGTGTTGGACATGCTGC |
| SD | TCCCAGCTCCAATAGTGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17646A-C11 | VGB6 | C57BL/6NTac | |
| 17646A-D2 | VGB6 | C57BL/6NTac | |
| 17646A-D10 | VGB6 | C57BL/6NTac | |
| 17646A-H7 | VGB6 | C57BL/6NTac | |
| 17646A-G7 | VGB6 | C57BL/6NTac | |
