| Gene | Ndufa10 |
| Chromosome | 1 |
| Deletion Start | 92,473,734 |
| Deletion End | 92,439,821 |
| Deletion Size | 33,914 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,473,427 | 92,473,446 | TTGTGGAACGCCTTGTAGAC |
| TUP | 92,473,397 | 92,473,419 | TGACGTGATGTGGAGCAGCAACA |
| TUR | 92,473,325 | 92,473,347 | GCACCTACTGTCACCCATTATTC |
| TDF | 92,440,599 | 92,440,616 | TGAGCCAGCTCCTCACAG |
| TDP | 92,440,573 | 92,440,597 | CTGCAGCACAAGAGTCGAGCCTTGA |
| TDR | 92,440,531 | 92,440,551 | TCTCCAGTTCTCATGCCCTTG |
PCR Primers
| Primer | Sequence |
| SU | ACACAACTCAAGCATCTGCG |
| SD | AAGTTGGAAGGGTTCAAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 122 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17651A-A11 | VGB6 | C57BL/6NTac | |
| 17651A-C8 | VGB6 | C57BL/6NTac | |
| 17651A-E3 | VGB6 | C57BL/6NTac | |
| 17651A-G11 | VGB6 | C57BL/6NTac | |
