| Gene | Nfe2 |
| Chromosome | 15 |
| Deletion Start | 103,251,039 |
| Deletion End | 103,248,407 |
| Deletion Size | 2,633 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,250,358 | 103,250,378 | CACCCAGGGAATGGCAAAGTC |
| TUP | 103,250,321 | 103,250,350 | TGACCCTTTGACTCTAGATAGCCACAGCAG |
| TUR | 103,250,239 | 103,250,259 | CAAGCCAAATGGCTCCTGTTC |
| TDF | 103,248,974 | 103,248,996 | GGCCCATCCCAACTATACTCTTC |
| TDP | 103,248,948 | 103,248,971 | CCCACTGAGACACCCTTGGCCTTA |
| TDR | 103,248,904 | 103,248,922 | CCACGGACAGCAGGCTTAG |
PCR Primers
| Primer | Sequence |
| SU | CCCAAACCTGAGAAAGACTGG |
| SD | CACCAATGAACTGAAGGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 140 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17653A-A5 | VGB6 | C57BL/6NTac | |
| 17653A-C11 | VGB6 | C57BL/6NTac | |
| 17653A-E7 | VGB6 | C57BL/6NTac | |
| 17653A-C3 | VGB6 | C57BL/6NTac | |
| 17653A-A8 | VGB6 | C57BL/6NTac | |
