| Gene | Tonsl |
| Chromosome | 15 |
| Deletion Start | 76,638,596 |
| Deletion End | 76,626,238 |
| Deletion Size | 12,359 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,638,079 | 76,638,099 | TGCCCGTGCTATGAAGATGAG |
| TUP | 76,638,051 | 76,638,076 | TCATGGAGAGTGAATGTTGCGTGCTA |
| TUR | 76,638,012 | 76,638,032 | AGCCCAAGGCGACGATGAAAG |
| TDF | 76,626,871 | 76,626,890 | TACACCAACGCACTCTCCAC |
| TDP | 76,626,844 | 76,626,866 | TAAGTGTGCCTGATGGCCTGGCC |
| TDR | 76,626,804 | 76,626,823 | GGCTGCCACCCTCAAATCTC |
PCR Primers
| Primer | Sequence |
| SU | TGACCACTGCCAATCAAGAG |
| SD | ACCTACTGAGACACTGGCCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17654A-A6 | VGB6 | C57BL/6NTac | |
| 17654A-B3 | VGB6 | C57BL/6NTac | |
| 17654A-B6 | VGB6 | C57BL/6NTac | |
| 17654A-B12 | VGB6 | C57BL/6NTac | |
| 17654A-D9 | VGB6 | C57BL/6NTac | |
