| Gene | Nol8 |
| Chromosome | 13 |
| Deletion Start | 49,678,466 |
| Deletion End | 49,653,649 |
| Deletion Size | 24,818 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 49,654,356 | 49,654,377 | CAACATCCATGCAAGGCAACAG |
| TUP | 49,654,381 | 49,654,406 | AATGAAGCGCCTCTTTGTGGGTGGAC |
| TUR | 49,654,463 | 49,654,485 | GTGATGATCTCCACGTCAGAAAC |
| TDF | 49,677,837 | 49,677,857 | ACTTCAGGCTTTCCAGAGCAG |
| TDP | 49,677,865 | 49,677,889 | TTACCTAGTGCTCAAGCAGGGAGCA |
| TDR | 49,677,893 | 49,677,915 | TGTCACACAATCACAGAAACCAC |
PCR Primers
| Primer | Sequence |
| SU | AGGCGGAGGAAATAAAGAGC |
| SD | GGTTTCATGTAAGCCTCCTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 130 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17655A-C4 | VGB6 | C57BL/6NTac | |
| 17655A-C5 | VGB6 | C57BL/6NTac | |
| 17655A-D8 | VGB6 | C57BL/6NTac | |
| 17655A-E8 | VGB6 | C57BL/6NTac | |
| 17655A-F2 | VGB6 | C57BL/6NTac | |
| 17655A-G3 | VGB6 | C57BL/6NTac | |
