| Gene | Nt5m |
| Chromosome | 11 |
| Deletion Start | 59,876,019 |
| Deletion End | 59,848,133 |
| Deletion Size | 27,887 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,848,657 | 59,848,680 | GAGATTGAACCCAGACCTCTGTAG |
| TUP | 59,848,683 | 59,848,706 | TAGACAGGCTCCCAACCACTGACC |
| TUR | 59,848,737 | 59,848,760 | GGGAAGCTAGTGAGAATGAAGGAC |
| TDF | 59,875,152 | 59,875,172 | AGGCACGGTGAGATTTGAGAG |
| TDP | 59,875,178 | 59,875,205 | TTAGTCAGTCCACACTGTTTCCTCCCAC |
| TDR | 59,875,214 | 59,875,234 | GTGTAGCAAACCCAGGCAGAG |
PCR Primers
| Primer | Sequence |
| SU | GTGAGCTCCTCCATCCAC |
| SD | CCAGAGCAAGAGACCGTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17659A-A4 | VGB6 | C57BL/6NTac | |
| 17659A-G7 | VGB6 | C57BL/6NTac | |
| 17659A-F4 | VGB6 | C57BL/6NTac | |
| 17659A-C7 | VGB6 | C57BL/6NTac | |
| 17659A-D5 | VGB6 | C57BL/6NTac | |
| 17659A-B4 | VGB6 | C57BL/6NTac | |
