Gene | Nt5m |
Chromosome | 11 |
Deletion Start | 59,876,019 |
Deletion End | 59,848,133 |
Deletion Size | 27,887 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 59,848,657 | 59,848,680 | GAGATTGAACCCAGACCTCTGTAG |
TUP | 59,848,683 | 59,848,706 | TAGACAGGCTCCCAACCACTGACC |
TUR | 59,848,737 | 59,848,760 | GGGAAGCTAGTGAGAATGAAGGAC |
TDF | 59,875,152 | 59,875,172 | AGGCACGGTGAGATTTGAGAG |
TDP | 59,875,178 | 59,875,205 | TTAGTCAGTCCACACTGTTTCCTCCCAC |
TDR | 59,875,214 | 59,875,234 | GTGTAGCAAACCCAGGCAGAG |
PCR Primers
Primer | Sequence |
SU | GTGAGCTCCTCCATCCAC |
SD | CCAGAGCAAGAGACCGTAGG |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 104 bp | + | + | - |
TDF | TDR | 83 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
17659A-A4 | VGB6 | C57BL/6NTac | |
17659A-G7 | VGB6 | C57BL/6NTac | |
17659A-F4 | VGB6 | C57BL/6NTac | |
17659A-C7 | VGB6 | C57BL/6NTac | |
17659A-D5 | VGB6 | C57BL/6NTac | |
17659A-B4 | VGB6 | C57BL/6NTac | |