| Gene | Palm |
| Chromosome | 10 |
| Deletion Start | 79,819,601 |
| Deletion End | 79,793,739 |
| Deletion Size | 25,863 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,794,126 | 79,794,144 | CTCCTCGTGGCCTCTTCTG |
| TUP | 79,794,147 | 79,794,164 | TCCGCAGCCTCTTGGCCA |
| TUR | 79,794,180 | 79,794,203 | CAGTGGCAACTCAGGACATCTAAG |
| TDF | 79,818,660 | 79,818,682 | AGCCCTGACCCTTCATTAGATTC |
| TDP | 79,818,684 | 79,818,706 | TGGAACCACCCTATCTGCGCCTC |
| TDR | 79,818,725 | 79,818,744 | CTCAGCTCTGCAAACTCCTC |
PCR Primers
| Primer | Sequence |
| SU | CTGCTGCTGTGGCTCTCTC |
| SD | ATAAGGCACAGACAGGCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17661A-B8 | VGB6 | C57BL/6NTac | |
| 17661A-H10 | VGB6 | C57BL/6NTac | |
