| Gene | Pipox |
| Chromosome | 11 |
| Deletion Start | 77,893,777 |
| Deletion End | 77,881,069 |
| Deletion Size | 12,709 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 77,893,178 | 77,893,197 | GGTAACAGCGACCTCCTCAC |
| TUP | 77,893,152 | 77,893,176 | TGATGCCCGGGAACTAAGACACAGG |
| TUR | 77,893,093 | 77,893,112 | CCCAGTGCCACCCTCATTTG |
| TDF | 77,881,611 | 77,881,633 | ACAGAGCCTGTAGTTCCTACCTG |
| TDP | 77,881,583 | 77,881,607 | TCAGAGGACACAGGTGTGACTCTCC |
| TDR | 77,881,510 | 77,881,529 | CAGCGCCGATGACAATGTTG |
PCR Primers
| Primer | Sequence |
| SU | AAATCAAGCTGCAGAGAGGG |
| SD | TTTGGCAAAGAAACCCAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 124 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17663A-A8 | VGB6 | C57BL/6NTac | |
| 17663A-A12 | VGB6 | C57BL/6NTac | |
| 17663A-G7 | VGB6 | C57BL/6NTac | |
| 17663A-F12 | VGB6 | C57BL/6NTac | |
| 17663A-E1 | VGB6 | C57BL/6NTac | |
| 17663A-D5 | VGB6 | C57BL/6NTac | |
