| Gene | Pmm2 |
| Chromosome | 16 |
| Deletion Start | 8,655,754 |
| Deletion End | 8,637,778 |
| Deletion Size | 17,977 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,638,068 | 8,638,087 | GCCCTCTGACCTGCTGTTTC |
| TUP | 8,638,093 | 8,638,114 | CCCAGCCCTGTCACCTTTGGCT |
| TUR | 8,638,131 | 8,638,153 | AGACGACTGAGAACCTGCCTTTG |
| TDF | 8,655,645 | 8,655,663 | TCCCTTGACCCGAGGAGTG |
| TDP | 8,655,664 | 8,655,691 | CCGGACTGACCAACAACACTGAAAGGTC |
| TDR | 8,655,732 | 8,655,751 | TGGCTCATTGGCAAGCACAG |
PCR Primers
| Primer | Sequence |
| SU | TGTCACCGGAAAGTTGAAGG |
| SD | CCAAAGCATAAGGGACCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17665A-A8 | VGB6 | C57BL/6NTac | |
| 17665A-F2 | VGB6 | C57BL/6NTac | |
| 17665A-E10 | VGB6 | C57BL/6NTac | |
