| Gene | Psma6 |
| Chromosome | 12 |
| Deletion Start | 55,418,396 |
| Deletion End | 55,398,919 |
| Deletion Size | 19,478 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,399,173 | 55,399,193 | GGCTGAAACCGAAGCGTAGTG |
| TUP | 55,399,195 | 55,399,217 | TCTTCAGGAGCGCCTTACTGGCC |
| TUR | 55,399,236 | 55,399,255 | GCGGGCATCACAGTGCAAAG |
| TDF | 55,417,726 | 55,417,750 | GGCTTTCTTCCTAGCTTAGGGATTG |
| TDP | 55,417,752 | 55,417,774 | AGGCAGGCAGTCGTGCCTTCCCT |
| TDR | 55,417,789 | 55,417,809 | GCATGAGCACAAGAACTGGAG |
PCR Primers
| Primer | Sequence |
| SU | TGGCCACTCTGATCTCCAG |
| SD | CAGCAGAGCACAATCCAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17671A-C12 | VGB6 | C57BL/6NTac | |
| 17671A-D10 | VGB6 | C57BL/6NTac | |
| 17671A-E5 | VGB6 | C57BL/6NTac | |
| 17671A-E7 | VGB6 | C57BL/6NTac | |
| 17671A-G9 | VGB6 | C57BL/6NTac | |
| 17671A-G11 | VGB6 | C57BL/6NTac | |
