| Gene | Rab1b |
| Chromosome | 19 |
| Deletion Start | 5,106,935 |
| Deletion End | 5,100,327 |
| Deletion Size | 6,609 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,106,356 | 5,106,373 | TGCTTGGGTCGTGCTGTC |
| TUP | 5,106,331 | 5,106,351 | TGCTCCCGGCGGTCTGTTCTT |
| TUR | 5,106,305 | 5,106,323 | GCCCGAGCAAAGGTGAGAG |
| TDF | 5,101,097 | 5,101,116 | GGTGTCACCTGCCTTTCTTC |
| TDP | 5,101,073 | 5,101,096 | TGATGTCCTTGGCTTCCCAGGAGA |
| TDR | 5,101,030 | 5,101,050 | CCTACAGTCATTGCTGCTTCC |
PCR Primers
| Primer | Sequence |
| SU | TGGCAAGTCGAATATCCATG |
| SD | GGAGTGAGGCAGAGATGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17674A-D2 | VGB6 | C57BL/6NTac | |
| 17674A-A6 | VGB6 | C57BL/6NTac | |
| 17674A-C6 | VGB6 | C57BL/6NTac | |
| 17674A-E9 | VGB6 | C57BL/6NTac | |
| 17674A-A3 | VGB6 | C57BL/6NTac | |
| 17674A-E12 | VGB6 | C57BL/6NTac | |
