| Gene | Rbm47 |
| Chromosome | 5 |
| Deletion Start | 66,027,255 |
| Deletion End | 66,019,141 |
| Deletion Size | 8,115 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 66,026,422 | 66,026,441 | GCGTGGAAAGGGTCAAGAAG |
| TUP | 66,026,398 | 66,026,421 | ATCCGTGACTACGCCTTCGTGCAC |
| TUR | 66,026,347 | 66,026,369 | TGTGCCGTTGAGGTTGTTCATAG |
| TDF | 66,019,626 | 66,019,647 | GCTTTGACAGGAGTCTGAACAC |
| TDP | 66,019,597 | 66,019,621 | AAGTTTGAGCGCATCAAGCCCTTCA |
| TDR | 66,019,543 | 66,019,565 | GCAGGTGAGTGACAGTACAGAAG |
PCR Primers
| Primer | Sequence |
| SU | ACTGGAGTCGAAAGTGCAGG |
| SD | ATAGTTCCCTAGATGCGCGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17675A-A7 | VGB6 | C57BL/6NTac | |
| 17675A-B4 | VGB6 | C57BL/6NTac | |
| 17675A-E10 | VGB6 | C57BL/6NTac | |
| 17675A-F4 | VGB6 | C57BL/6NTac | |
| 17675A-F10 | VGB6 | C57BL/6NTac | |
| 17675A-H1 | VGB6 | C57BL/6NTac | |
