17676 Rbpj Status: Cancelled
| Gene | Rbpj |
| Chromosome | 5 |
| Deletion Start | 53,627,415 |
| Deletion End | 53,590,353 |
| Deletion Size | 37,063 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,590,431 | 53,590,450 | GACTACTCGGAGGGCTTGTC |
| TUP | 53,590,459 | 53,590,479 | AGCGGCCTGCGCATGCTCCAT |
| TUR | 53,590,500 | 53,590,521 | AGGGATCTGCTCACTGCCTTTC |
| TDF | 53,626,703 | 53,626,726 | CATTCCATGTCCCTATTGCCTTAC |
| TDP | 53,626,736 | 53,626,761 | AGTGGGATTCCTGCAGACGAACCCAG |
| TDR | 53,626,771 | 53,626,792 | AACTGCTCAGTGGTAGCATGTG |
PCR Primers
| Primer | Sequence |
| SU | GGGAGCAAATGAATGGAGAC |
| SD | TCCCTTACACATCAAAGTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
