| Gene | Recql |
| Chromosome | 6 |
| Deletion Start | 142,384,541 |
| Deletion End | 142,362,693 |
| Deletion Size | 21,849 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 142,383,969 | 142,383,988 | AGGGTTACAACAGCAAGCAC |
| TUP | 142,383,941 | 142,383,967 | ACTAGTCACTCGCCATCTCCCTAGTTC |
| TUR | 142,383,913 | 142,383,934 | AGGTCCCATTCTAGGGATACAC |
| TDF | 142,363,641 | 142,363,660 | ACGGCTTACGCCACCATCTC |
| TDP | 142,363,613 | 142,363,638 | ATCTGAAGGTGGGACCCAGAGCTTGT |
| TDR | 142,363,562 | 142,363,584 | TGCTGACTTCTTCACTTGCATAG |
PCR Primers
| Primer | Sequence |
| SU | AAACGATGCTCTCCAGGAAC |
| SD | GCTCCTCTGTTTGCCTGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17677A-B10 | VGB6 | C57BL/6NTac | |
| 17677A-D9 | VGB6 | C57BL/6NTac | |
| 17677A-G10 | VGB6 | C57BL/6NTac | |
| 17677A-E12 | VGB6 | C57BL/6NTac | |
