| Gene | Rnf126 |
| Chromosome | 10 |
| Deletion Start | 79,766,824 |
| Deletion End | 79,759,008 |
| Deletion Size | 7,817 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,765,894 | 79,765,916 | GGTGGTAAGAGGTGCTGTAGTTC |
| TUP | 79,765,867 | 79,765,891 | TCTGGTCTCTGCACTCTCCCTCTGG |
| TUR | 79,765,829 | 79,765,851 | GGAGTTAATCGCCCTGAAGAATG |
| TDF | 79,759,522 | 79,759,542 | GCCTCTGACGTACACCTGAAC |
| TDP | 79,759,499 | 79,759,520 | TCCTTCACTGTGGCCCTCCCTG |
| TDR | 79,759,437 | 79,759,456 | CTGTATGGCTGCGGCTCAAG |
PCR Primers
| Primer | Sequence |
| SU | ACACACTGGACCCAGGTCTCT |
| SD | TTGTCTGCTGGAGCAGTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17678A-G5 | VGB6 | C57BL/6NTac | |
| 17678A-A7 | VGB6 | C57BL/6NTac | |
| 17678A-G7 | VGB6 | C57BL/6NTac | |
