| Gene | Sar1b |
| Chromosome | 11 |
| Deletion Start | 51,791,461 |
| Deletion End | 51,777,507 |
| Deletion Size | 13,955 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,778,231 | 51,778,250 | TAGTTGCTCAGGCTGGCTTG |
| TUP | 51,778,253 | 51,778,279 | ACTCATGTTCTTCCTGGCTCTGCCTCC |
| TUR | 51,778,296 | 51,778,318 | GCTGGGTTTCATAGCCATTTCTG |
| TDF | 51,790,868 | 51,790,888 | TAGGCCGTGGTCTCATTACAG |
| TDP | 51,790,892 | 51,790,915 | AGGCTGGCCTTGCACTTTGATCCC |
| TDR | 51,790,918 | 51,790,935 | CACGCAATCCCAGCACTC |
PCR Primers
| Primer | Sequence |
| SU | GGGACTTGTTGTTTATGTATGCAC |
| SD | AAGGCATCATGCAATCACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17681A-D3 | VGB6 | C57BL/6NTac | |
| 17681A-E4 | VGB6 | C57BL/6NTac | |
| 17681A-E5 | VGB6 | C57BL/6NTac | |
| 17681A-G1 | VGB6 | C57BL/6NTac | |
| 17681A-H7 | VGB6 | C57BL/6NTac | |
| 17681A-H8 | VGB6 | C57BL/6NTac | |
