| Gene | Smarcal1 |
| Chromosome | 1 |
| Deletion Start | 72,616,574 |
| Deletion End | 72,585,699 |
| Deletion Size | 30,876 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,586,307 | 72,586,327 | GGGAGCGTGCATAAAGACTGG |
| TUP | 72,586,330 | 72,586,356 | ATCGCTTCCGGGTAAAGATCGGCTACA |
| TUR | 72,586,374 | 72,586,394 | GCTGGGCAGAGACTTAAACAC |
| TDF | 72,616,030 | 72,616,051 | GTGGATGCTGGGAATCAAAGTG |
| TDP | 72,616,056 | 72,616,082 | CCTCTGCATGAGCAACAAATGCTCTGA |
| TDR | 72,616,101 | 72,616,121 | GAACGAGGAGTGGCAAGGATG |
PCR Primers
| Primer | Sequence |
| SU | TGGCTAGCAAATGAGAAGGG |
| SD | TCAGGATTGCCTCAGGTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17683A-A9 | VGB6 | C57BL/6NTac | |
| 17683A-B9 | VGB6 | C57BL/6NTac | |
| 17683A-D5 | VGB6 | C57BL/6NTac | |
| 17683A-F9 | VGB6 | C57BL/6NTac | |
| 17683A-G1 | VGB6 | C57BL/6NTac | |
