| Gene | Snap29 |
| Chromosome | 16 |
| Deletion Start | 17,428,301 |
| Deletion End | 17,406,126 |
| Deletion Size | 22,176 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,406,724 | 17,406,744 | TGGCCCAGTTAAAGTGTGAAC |
| TUP | 17,406,745 | 17,406,770 | TGTGTATGCTTCAAGTGCCAGGTGGT |
| TUR | 17,406,781 | 17,406,800 | ACAGGCTCCCTTTGCGTCAG |
| TDF | 17,427,764 | 17,427,783 | AGCATGTGCAAGGCTCTGTG |
| TDP | 17,427,788 | 17,427,810 | ATTCTCCAGCATGCAGGGCGGAA |
| TDR | 17,427,848 | 17,427,867 | AGCCCTCCCTGCTTCTTCTG |
PCR Primers
| Primer | Sequence |
| SU | AAAGCCTCGAGAGGTGCTG |
| SD | CAGTAAGGCCTCGGAGTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17685A-A9 | VGB6 | C57BL/6NTac | |
| 17685A-A10 | VGB6 | C57BL/6NTac | |
| 17685A-A12 | VGB6 | C57BL/6NTac | |
| 17685A-C11 | VGB6 | C57BL/6NTac | |
| 17685A-E2 | VGB6 | C57BL/6NTac | |
| 17685A-H9 | VGB6 | C57BL/6NTac | |
