| Gene | Snap47 |
| Chromosome | 11 |
| Deletion Start | 59,438,471 |
| Deletion End | 59,407,581 |
| Deletion Size | 30,891 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,438,228 | 59,438,247 | CAAGCACTGGTTCAGCTCAC |
| TUP | 59,438,201 | 59,438,227 | TGCAGCCTAGTCGAAATGTGGTCTTCA |
| TUR | 59,438,170 | 59,438,189 | AGCAGCTCCCTCCAGAAATG |
| TDF | 59,408,063 | 59,408,085 | TCCCACAGTTCATGGGTGTATTC |
| TDP | 59,408,039 | 59,408,061 | TGGCCAGGTCCACATGGGTTTCA |
| TDR | 59,408,019 | 59,408,038 | ATGCCCTGGAAGGTGCTGAG |
PCR Primers
| Primer | Sequence |
| SU | GGACCCTTAGGAGGACCTTG |
| SD | GCACACACAGAGGCTACTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17686B-B5 | VGB6 | C57BL/6NTac | |
| 17686B-C3 | VGB6 | C57BL/6NTac | |
| 17686B-E6 | VGB6 | C57BL/6NTac | |
| 17686B-E10 | VGB6 | C57BL/6NTac | |
| 17686B-F6 | VGB6 | C57BL/6NTac | |
| 17686B-H5 | VGB6 | C57BL/6NTac | |
