17687   Socs7     Status: ES cell colonies screened / QC positives

GeneSocs7
Chromosome11
Deletion Start97,389,729
Deletion End97,362,645
Deletion Size27,085
Genome Build38
MGI IDMGI:2651588
NCBI Gene ID192157
BAC ID264I2
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF97,363,45297,363,473GCAAGCGACAAAGTTCCATTTC
TUP97,363,47597,363,501TGTTTCGTTTCCCTCGTTTTCCCCTAC
TUR97,363,51597,363,535TCCACAGCTCAGCTCTAACAG
TDF97,389,10697,389,124TTGAAGGACTGCCACCAAC
TDP97,389,12597,389,152TCCAGTACAGCTGCTCTATCCAGTGTCC
TDR97,389,15497,389,176GAGGGACTTGACATTGCTGAATC

PCR Primers

PrimerSequence
SUTCCTGAGCCGCCTCCTCG
SDAACTTCCAAGTGGATCACAGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR84 bp++-
TDFTDR71 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17687A-C4VGB6C57BL/6NTac
17687A-F9VGB6C57BL/6NTac
17687A-F5VGB6C57BL/6NTac
17687A-C3VGB6C57BL/6NTac
17687A-G10VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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