| Gene | Tbc1d10c |
| Chromosome | 19 |
| Deletion Start | 4,190,806 |
| Deletion End | 4,184,634 |
| Deletion Size | 6,173 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,190,329 | 4,190,348 | TCAGGACTTCAGGCTTCCAG |
| TUP | 4,190,305 | 4,190,328 | TCCAGGGCACTATCCACCCAGCTT |
| TUR | 4,190,232 | 4,190,256 | TGGGATGAGGAGGTATTTAGGGTAG |
| TDF | 4,185,113 | 4,185,135 | CAAGGCTTCCTACTTCCTGACAC |
| TDP | 4,185,086 | 4,185,112 | CCCAGTCCAGGCAAAGTTAGCATCAAC |
| TDR | 4,185,048 | 4,185,066 | CGCTCAGACAGGGTTACAC |
PCR Primers
| Primer | Sequence |
| SU | GCAAGCAGGAAGAACCTCAC |
| SD | GGCTCCCTTTCCCTGTAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 117 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17689A-C8 | VGB6 | C57BL/6NTac | |
| 17689A-F12 | VGB6 | C57BL/6NTac | |
| 17689A-E7 | VGB6 | C57BL/6NTac | |
| 17689A-H9 | VGB6 | C57BL/6NTac | |
