| Gene | Tcf7 |
| Chromosome | 11 |
| Deletion Start | 52,282,214 |
| Deletion End | 52,253,796 |
| Deletion Size | 28,419 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,281,524 | 52,281,544 | GGCGACACGGCTGTATTCATC |
| TUP | 52,281,493 | 52,281,518 | CCATGGCTTTGGCCCTTCAAGGCAAG |
| TUR | 52,281,456 | 52,281,475 | GCTGCTACCCAGCTCAGAAC |
| TDF | 52,254,154 | 52,254,176 | AAGATTCCTCTTGCCACCTTGTG |
| TDP | 52,254,122 | 52,254,149 | TCCCGTGTAATTGCATTTGTCCTAGGTC |
| TDR | 52,254,095 | 52,254,113 | GGCCATCTTCCAGCCCTAC |
PCR Primers
| Primer | Sequence |
| SU | TCGCTGTTCCCTGTCAAGTC |
| SD | GAGGTCCAGCCAGCAATAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17690A-H9 | VGB6 | C57BL/6NTac | |
| 17690A-A9 | VGB6 | C57BL/6NTac | |
| 17690A-B3 | VGB6 | C57BL/6NTac | |
| 17690A-H3 | VGB6 | C57BL/6NTac | |
| 17690A-D7 | VGB6 | C57BL/6NTac | |
| 17690A-A2 | VGB6 | C57BL/6NTac | |
