| Gene | Tfam |
| Chromosome | 10 |
| Deletion Start | 71,237,937 |
| Deletion End | 71,228,554 |
| Deletion Size | 9,384 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,237,547 | 71,237,569 | GTCACCCATGATGCTAGGACTTG |
| TUP | 71,237,515 | 71,237,540 | CCCTCTTTGGTTTGGCTAGGTCACCC |
| TUR | 71,237,475 | 71,237,494 | AAGAGGTTGCAGGGCTGAGG |
| TDF | 71,229,486 | 71,229,506 | AGGCCCAAGGGATGGAAAGTG |
| TDP | 71,229,462 | 71,229,484 | CCTCTTTCCCAGGTCACCGCTCA |
| TDR | 71,229,434 | 71,229,455 | TTAATTCTCCTCAGCCCACCAG |
PCR Primers
| Primer | Sequence |
| SU | CTCCCTAGCCTCAGGCTC |
| SD | GGGCCACACCCATATGTAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17691A-G12 | VGB6 | C57BL/6NTac | |
| 17691A-C9 | VGB6 | C57BL/6NTac | |
| 17691A-F3 | VGB6 | C57BL/6NTac | |
| 17691A-D5 | VGB6 | C57BL/6NTac | |
| 17691A-E3 | VGB6 | C57BL/6NTac | |
| 17691A-C10 | VGB6 | C57BL/6NTac | |
