| Gene | Tnfaip1 |
| Chromosome | 11 |
| Deletion Start | 78,530,229 |
| Deletion End | 78,525,303 |
| Deletion Size | 4,927 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,529,435 | 78,529,455 | TGAAGATAGCAGTTCCCAGAC |
| TUP | 78,529,408 | 78,529,434 | TCAGGATAGGGAAGAGTAGCACTGTCC |
| TUR | 78,529,351 | 78,529,371 | CCACATGAGGAGAGGCCAGAG |
| TDF | 78,525,758 | 78,525,776 | CAGCCCAGAAAGCTCAGAC |
| TDP | 78,525,732 | 78,525,755 | CATCTGGCACCAAGCCCATGGAGT |
| TDR | 78,525,670 | 78,525,691 | CTACCCTACAGCATCCCACAAC |
PCR Primers
| Primer | Sequence |
| SU | GTACAGTGAGGAAGGGCTTC |
| SD | AACAAAGCTGTTCTCGTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17692A-B5 | VGB6 | C57BL/6NTac | |
| 17692A-E12 | VGB6 | C57BL/6NTac | |
| 17692A-B2 | VGB6 | C57BL/6NTac | |
| 17692A-F9 | VGB6 | C57BL/6NTac | |
| 17692A-E6 | VGB6 | C57BL/6NTac | |
| 17692A-G5 | VGB6 | C57BL/6NTac | |
