| Gene | Tnip2 |
| Chromosome | 5 |
| Deletion Start | 34,513,894 |
| Deletion End | 34,496,595 |
| Deletion Size | 17,300 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,513,420 | 34,513,441 | GGAGCTGGGTTATGAGGGATAC |
| TUP | 34,513,387 | 34,513,414 | CCTGAGACTAGTGGGTCCTGAGGTTTCA |
| TUR | 34,513,344 | 34,513,365 | AACAGGATTACCTGCGCCTCTG |
| TDF | 34,497,491 | 34,497,509 | GGCTGCCACTGTAGGTCTC |
| TDP | 34,497,464 | 34,497,488 | AGGCTGTCTAGCACCTCGAATCGCA |
| TDR | 34,497,426 | 34,497,447 | ACCAGGGAACAGGCCAACTAAG |
PCR Primers
| Primer | Sequence |
| SU | AGCCAATGAAACCTGACCAC |
| SD | CTCTGCTGAGTTTGGGAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17693A-F7 | VGB6 | C57BL/6NTac | |
| 17693A-G9 | VGB6 | C57BL/6NTac | |
| 17693A-B8 | VGB6 | C57BL/6NTac | |
