| Gene | Tpi1 |
| Chromosome | 6 |
| Deletion Start | 124,814,119 |
| Deletion End | 124,811,257 |
| Deletion Size | 2,863 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,813,529 | 124,813,551 | TTCCACATCCCTATCCTTCACAG |
| TUP | 124,813,507 | 124,813,528 | CATGGGCCACTCCTGCCTTCCA |
| TUR | 124,813,466 | 124,813,485 | CCAACGGCTCAGCATGTTTG |
| TDF | 124,812,208 | 124,812,228 | GGTCTGTGGGTAAAGCCTGAG |
| TDP | 124,812,177 | 124,812,203 | TGAGTTTGAGTCCTCCAAAGACAGCCA |
| TDR | 124,812,133 | 124,812,154 | GAGGGCAGAGGACAACTTGTAG |
PCR Primers
| Primer | Sequence |
| SU | GACGTTGGTGACACACTTGG |
| SD | CTATCCCAGGCCTCTTAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17694A-C7 | VGB6 | C57BL/6NTac | |
| 17694A-H3 | VGB6 | C57BL/6NTac | |
| 17694A-D12 | VGB6 | C57BL/6NTac | |
| 17694A-F1 | VGB6 | C57BL/6NTac | |
| 17694A-F9 | VGB6 | C57BL/6NTac | |
| 17694A-G3 | VGB6 | C57BL/6NTac | |
