| Gene | Trim66 |
| Chromosome | 7 |
| Deletion Start | 109,486,052 |
| Deletion End | 109,453,935 |
| Deletion Size | 32,118 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,485,152 | 109,485,175 | GACATCTTCCTCTGCTGTAACTTC |
| TUP | 109,485,125 | 109,485,148 | TCATCTACCTCCTCGCCATCAGCA |
| TUR | 109,485,084 | 109,485,101 | GGCTGGGCACTTTCTCAC |
| TDF | 109,454,342 | 109,454,363 | GCCAAGGCTGATTGGAACTAAC |
| TDP | 109,454,305 | 109,454,334 | ATCAGGATGGCCTTAAACTTCTTCCTCCCA |
| TDR | 109,454,261 | 109,454,281 | CAGAATGGCTGCACGGAGTAG |
PCR Primers
| Primer | Sequence |
| SU | CTAGACCGGTGGTCTTGAGG |
| SD | ACACAAGGTGTTCCTGAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17696A-F12 | VGB6 | C57BL/6NTac | |
| 17696A-G8 | VGB6 | C57BL/6NTac | |
| 17696A-A10 | VGB6 | C57BL/6NTac | |
| 17696A-G6 | VGB6 | C57BL/6NTac | |
